Steps of a Typical SPR Experiment

1) Instrument Cleaning

The importance of starting an experiment with a clean instrument cannot be overemphasized (see Myszka, D. G. Improving Biosensor Analysis. J. Mol. Recognition. 12: 279-284; (1999). A typical cleaning procedure consists of consecutive injections of the following solutions:

Procedure:

1. Flush with water
2. Flush with 20% ethanol
3. Flush with water
4. Flush with 0.5% SDS
5. Flush with 50mM glycine pH 9.5
6. Flush thoroughly with water

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