Features:
- Simultaneous 2 Channel Surface Plasmon Resonance Measurements
- Reference Channel Minimizes Non-Specific Binding and Bulk Refractive Index Effects
- Can be Fully Automated with Addition of an Autosampler
- System is HPLC fluidics based
a) User serviceable
b) Nano to micro to macro applications
c) 0.0025" to 0.04" I.D. Tubing
d) Allows flow of cell lysates
- Sapphire Prism
a) Virtually indestructible
b) High thermal conductivity contributes to exceptional thermal stability
- Low Molecular Weight Analyte Detection Limits
- 10 Deg. C. to 90 Deg. C. Temperature Range
a) Temperature changes programmable in user defined steps and intervals
b) Change temperature in minutes not hours
c) Kinetic capability over a broad temperature range – determine ΔH
- High Speed Scanning for Fast Kinetics
- Sensitivity: 0.35 µ Refractive Index Units rms (0.25 RU)
- Low Drift for Long Association/Dissociation Times, < 0.1 μRIU/min (1 μRIU = 0.73 RU = 7.3 e-05°)
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Applications:
- Widely used for, but not limited to, determining interactions between DNA oligomers, RNA, peptides, proteins,
- Protein/Peptide Binding to lipids/vesicles with or without incorporated proteins
- Kinetic rate constants (on-forward-association, off-reverse-dissociation)
- Thermodynamics (Association and Dissociation Equilibrium Constants)
- Concentration monitoring
- Sequence recognition, Epitope screening and mapping
- Method development
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