Surface Plasmon Resonance Software

Software

Data Acquisition and Alignment

SPR_V4017 Data Acquisition and Alignment Program

The software collects refractive index response, temperature and timed event marks vs. time data from the SR77000 or SR7000DC. This program is also used to set instrument operating parameters. Files are tab delimited text files and can be opened in standard spreadsheet programs. The data acquisition program displays each channel individually, both channels or the difference between channels. The data acquisition interface is shown in Figure 1A. This program is also used to extract and overlay a series of response vs. time curves – see Figure 1B. Overlaid / normalized Data can then be saved as a tab delimited file in formats compatible with global fitting programs “Clamp” or “Scrubber™” (see Biologic Software and The Center for Biomolecular Interaction Analysis http://www.cores.utah.edu/interaction/). Data is also easily imported into standard analysis programs, e.g. Graphpad Prism™, Origin™ or CurveExpert™. Figures 2 a - c show typical data analysis steps using “Scrubber™“ for the interaction of human serum albumin (HSA) with immobilized anti-HSA IgG.

SPR_V4017 also controls the SR7300 semi-automatic injection valve.

Figure 1A: SPR_V4017 data acquisition interface		Figure 1B: SPR_4017 “Load and Align” form

Data Analysis – Scrubber 2^®

Links:

Scrubber 2 Software

Additional information on Biomolecular Interactions

Tutorial on theory of data analysis

Scrubber 2 is a commercial program of Biologic Software. The program can read and write the SR7000 and SR7000DC data files. The purpose of the program is to align and clean the response vs. time data and globally fit data to biointeraction models to determine reaction kinetics and/or equilibrium constants. Scrubber critically aligns response curves and renormalizes the response and time scales to zero. It can remove bulk refractive index shifts from the SR7000 single channel SPR Spectrometer. Scrubber allows users to set separate start and stop times for each injection – this is important especially with manual systems where start and stop times may vary slightly. It can also correct for bulk refractive index shifts introduced by use of DMSO to dissolve samples that are not easily dissolved in running buffer alone. It also subtracts reference channel response (SR7000DC) and buffer blanks.

Figure 2A: Raw overlaid data		Figure 2B: Time zeroed, aligned, referenced and buffer blank subtracted data
Figure 2C: Global data fit to 1:1 binding model

Our software precisely controls the Injection (Association) and Load (Dissociation) times for all sample injections. Injection Volumes, the Syringe speed (you adjust slower for more viscous samples), the needle height and whether or not the sample is picked up using headspace pressure and an air segment can also be controlled by the user.

The software can be programmed to refill the syringe on a syringe pump for continuous overnight sampling. A separate Maintenance menu allows the user to perform needlewashes.

Systems, Methods and Run Tables can be created, saved, and exported and imported from one system or computer to another.